Effect of Osmosis Time and Sucrose Concentration on The Antioxidant Activity of Red Dragon Fruit Juice (Hylocereus polyrhizus)
Efek Lama Osmosis dan Konsentrasi Sukrosa Terhadap Aktivitas Antioksidan (DPPH) Sari Buah Naga Merah (Hylocereus polyrhizus)
Abstract
Dragon fruit contains antioxidants, beta-carotene, ascorbic acid and dietary fiber content in the form of pectin. Antioxidant are chemical compounds that can donate one or more electrons to free radicals to inhibit free radical reactions. Red dragon fruit can be processed into natural red dragon fruit juice using osmosis extraction. Osmotic extraction is done by soaking the fruits with sugar, it is done so that the water comes out towards the media. The advantages of osmosis are also many of them are easy, natural manufacture, the tools used are simple so that the fruit juice produced is safe for consumption by humans. The purpose of this study to determine the antioxidant activity of red dragon fruit juice. Antioxidant activity testing was carried out using the DPPH method (1,1 Diphenyl-2-picrylhydrazyl). This research was conducted using a factorial Randomized Block Design (RAK) with the first factor being sugar concentration which consisted of 3 treatment levels, namely (25%, 50% and 75%) and the second factor was osmosis duration (12, 24, and 36 hours). The results showed that red dragon fruit juice in the 24-hour 50% treatment had the highest antioxidant activity with an IC50 value of 28.
References
Badarinath A, Rao K, Chetty CS, Ramkanth S, Rajan T, & Gnanaprakash K. (2010). A Review on In-vitro Antioxidant Methods: Comparisons, Correlations, and Considerations. International Journal of PharmTech Research, 2010:1276-1285.
Farikha, I. N., Anam, C., & Widowati, E. (2013). Pengaruh jenis dan konsentrasi bahan penstabil alami terhadap karakteristik fisikokimia sari buah naga merah (Hylocereuspolyrhizus) selama penyimpanan. Jurnal Teknosains Pangan, 2(1).
Fukumoto, LR dan Mazza G. (2000). Assesing antioxidant andprooxidant activities of phenolic compounds. J agric food 48 (8):3597-3604.
Molyneux, P. (2004). The use of the stablefree radikal diphenyl picrylhydrazyl (DPPH) forestimating antioxidant activity. Journal Science of Technology 26(2):211-219.
Muas, I. and Jumjunidang. (2015). Status of dragon fruit cultivation and marketing in Indonesia. Workshop on improving pitaya production and marketing. International workshop proceedings. 7-9 September 2015. Fengshan, Kaohsiung, Taiwan. p. 19-29.
Mukarommah, U., Sri, H.S., Siti, A. (2010). Kadar Vitamin C, Mutu Fisik, pH Dan Mutu Organoleptik Sirup Rosella (Hibiscus sabdariffa, L) Berdasarkan Cara Ekstraksi. Jurnal Pangan Dan Gizi Vol. 01 No. 01.
Pratomo. (2008). Superioritas Jambu Biji dan Buah Naga. http://www.unika.ac.id/pasca/pmpt/?p=5. (Diakses pada tanggal 12 Agustus 2011).
Prayoga G. (2013). Fraksinasi, Uji Aktivitas Antioksidan dengan Metode DPPH dan Identifikasi Golongan Senyawa Kimia dari Ekstrak Teraktif Daun Sambang Darah (Excoecaria cochinchinensis Lour). Fakultas Farmasi. Program Studi Sarjana Ekstensi Universitas Indonesia.
Robinson, T., (1983). The Organic Constituents of Higher Plants Their Chemistry and Interrelationships, 5th Ed., 200, Cordus Press., North Amherst.
Saputra, D. (2006). Osmosis-Puffing Sebagai Suatu Alternatif Proses Pengeringan Buah dan Sayur. Keteknikan Pertanian Vol. 20 No. 1.
Winarsi, H. (2007). Antioksidan Alami dan Radikal Bebas. Yogyakarta: Penerbit Kanisius.
Copyright (c) 2023 Idha Noer Azizah, Rahmah Utami Budiandari, Al Machfudz WDP
This work is licensed under a Creative Commons Attribution 4.0 International License.