Total Polyphenol Content of Bromelia Leaves (Neoregelia marmorata) Etanol Extract
Kandungan Polifenol Total Ekstrak Etanol Daun Bromelia (Neoregelia marmorata)
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(1) * Malikah Safitri
Akademi Analis Farmasi Dan Makanan Putra Indonesia Malang
Indonesia
(2) Erna Susanti Akademi Analis Farmasi Dan Makanan Putra Indonesia Malang
Indonesia
(*) Corresponding Author
Abstract
This study aims to determine total polyphenol content of Bromelia (Neoregelia marmorata) leaves extract with ethanol and water solvent ratio. There are three kinds of comparisons, ethanol 96%, ethanol 50 % and aquadest. The third variation of the extract is then determined the total polyphenol content. Determination of total polyphenol content was performed using visible light spectrophotometry at wavelength 758 nm with Folin-ciocalteu reagent and standard used was gallic acid. The highest average polyphenol content was obtained ethanol extract 96% then ethanol 50%, aquadest that is 0,4710 mgGAE / g; 0.3677 mgGAE / g; 0.1847 mgGAE / g. The more part of ethanol 96%, the total polyphenol content is higher. Furthermore, one way anova test is used to know the difference in the three extracts and followed by Post Hoc test. The results of the analysis showed significant differences between the three variants extract with probability value (p≤0,05).
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Alothman, M., Bhat, R., Karim, A.A., 2009. Antioxidant Capacity And Phenolic Content of Selected Tropical Fruits from Malaysia, Extracted with Different Solvent. Food Chem. 115, 785-788.
Apsari, P.D., dan Hari Susanti. 2011. Perbandingan Kadar Fenolik Total Ekstrak Methanol Kelpoak Merah dan Ungu Bunga Rosella (Hibiscus sabdariffa, Linn) Secara Spektrofotometri. ISBN: 978-979-18458-4-47.
Bajcan, D., et al. 2013. Optimizing Condition for Spectrophotometric Determination of Total Poliphenols on Wines Using Folin-Ciocalteu Reagent. J. Microbiol biotechnol food sci. 2(1): 1699-1708.
Brewer, M.S. 2011. Natural Antioxidationts: Sources, Compounds, Mechanism of Action and Potential Application. Comp. Rev. Food Scie. Food Saf. 10:221-247.
Coelho, R G et al. 2010. “Chemical Composition and Antioxidant and Antimycobacterial Activities of Bromelia Balansae (Bromeliaceae).” Journal of Medicinal Food 13(October 2010): 1277–80.
Dai, Jin, and Russell J Mumper. 2010. “Plant Phenolics: Extraction, Analysis and Their Antioxidant and Anticancer Properties.” : 7313–52.
Direktorat Pengawasan Obat Tradisional. 2000. Parameter Standar Umum EKstrak Tumbuhan Obat. Jakarta: Departemen Kesehatan Republik Indonesia.
Folin dan Ciocalteu. 1994. On tyrosine and trythophane determination in proteins. Jour. Bio. Chem. 73: 627-650, 1927, in Todd-Standford, 10, 412.
Gonçalves, Raimundo et al. 2012. “Phytochemical Screening , Antioxidant and Antibacterial Activity of Extracts from the Flowers of Neoglaziovia Variegata ( Bromeliaceae ).” 4(10): 4489–94.
Harborne, J.R. 1987. Metode Fitokimia Penuntun Cara Modern Menganalisis Tumbuhan. cetakan II. Terjemahan oleh Kosasih Paadmawinata. Bandung: ITB.
Koffi, E, et al. 2010. Effect of Solvent Type On Extraction Of Polyphenols From Twenty Three Ivorian Plants. Journal of Animal and Plants Sciences Vol 5 Issue 3:550-558.
Kresnawaty, I dan Zainuddin, A. 2009. Aktivitas antioksidan dan atibakteri dari derivat metil ekstrak etanol daun gambir (Uncaria gambir). Jurnal Littri. 15(4);145-151.
Lafka, T.I., Sinanoglou, V., Lazos, E.S., 2007. On Extraction and Antioxidant Activity of Phenolic Compound from Winery Wastes. Food Chem. 104 (3), 1206-1214.
Marjoni, Mhd Riza, Afrinaldi, and Ari Devi Novita. 2015. “Kandungan Total Fenol Dan Aktivitas Antioksidan Ekstrak Air Daun Kersen ( Muntingia Calabura L .).” Jurnal Kedokteran Yarsi 23(2): 187–96.
Nunes, X.P., 2012. Biological Oxidation and atioxidation activity of natural produsts. university Federal Sao Fransisco. Brazil. pp 1-20.
Raimundo, G. De Oliveira-Jnior et al. 2014. “The First Flavonoid Isolated from Bromelia Laciniosa (Bromeliaceae).” Journal of Medicinal Plants Research 8(14): 558–63. http://academicjournals.org/journal/JMPR/article-abstract/0CE864E43945.
Robinson, Trevor. 1955. Kandungan Organik Tumbuhan Tinggi. Bandung: ITB.
Sayuti, Kesuma, and Rina Yenrina. 2015. Antioksidan Alami Dan Sintetik. Pertama. Padang: Andalas University Press.
Senja, Rima Yulia, et al. 2014. Perbandingan metode ekstraksi dan variasi pelarut terhadap rendemen dan aktivitas antioksidan ekstrak kubis ungu (Brassica oleracea L. var.capitata f. rubra). Traditional Medicine journal 19(1) 43-48.
Suryani, Ch Lilis. 2012. “Optimasi Metode Ekstraksi Fenol Dari Rimpang Jahe Emprit (Zingiber Officinalle Var, Rubrum).” Jurnal AgriSains 3(4): 63–70.
Ukieyanna, Elsha. 2012. Aktivitas antioksidan, kadar fenolik, dan flavonoid total tumbuhan suruhan (peperomia pellucida L. kunth). Bogor:Institute Pertanian Bogor.
Vatai, T.; Skerget, M.; Knez, Z. 2009 Extraction of Phenolic Compounds From Elder Berry and Differentgrape Marc Varieties Using Organic Solvents And/Or Supercritical Carbon Dioxide. J. Food Eng.
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Published
2023-01-25
How to Cite
Safitri, M., & Susanti, E. (2023). Total Polyphenol Content of Bromelia Leaves (Neoregelia marmorata) Etanol Extract . Journal of Tropical Food and Agroindustrial Technology, 4(01), 13-19. https://doi.org/10.21070/jtfat.v4i01.1609
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Articles
Copyright (c) 2023 Malikah Safitri, Erna Susanti
This work is licensed under a Creative Commons Attribution 4.0 International License.
